Molecular Devices

Application Note: Improved cell counting method using transmitted light

Hematoxylin is the most commonly used dye for staining protocols in histology. It reacts like a basic dye with a purplish-blue color and stains acidic or basophilic structures, including the cell nucleus and organelles that contain RNA, such as ribosomes and the rough endoplasmic reticulum.

Here, we demonstrate how hematoxylin can be used on the SpectraMax® i3x microplate reader with the SpectraMax® MiniMax™ cytometer to increase the contrast in transmitted light images to improve cell counting for cell lines with relatively flat morphology or indistinguishable intercellular space.

 

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