Molecular Devices

Confident assurance of clonality using calcein AM with minimal effect on viability

The development of cell lines that express a specified protein of interest is critical to the generation of biopharmaceutical molecules such as monoclonal antibodies.  To establish a cell line, single cells are seeded in wells and allowed to proliferate to form a colony, which can be further expanded after assessing productivity.

Instrument technologies exist to seed single cells in a microplate format, including flow cytometry and single cell printing devices. A more traditional approach to obtain single-cell wells is known as limiting dilution. In this technique, a series of serial dilutions arrives at single cell wells along with a distribution of void wells and multiple-cell wells at a predicted statistical probability.

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