Molecular Devices

Application Note: SNP Genotyping with FRET Based KASP Technology on SpectraMax Microplate Readers

Genotyping is a process for analyzing genetic differences among individuals by examining their DNA sequences. The many methods available for genotyping enable researchers to investigate genomic diversity in humans, microorganisms, and plants. Single nucleotide polymorphisms (SNPs) are one of the most common types of genetic variation, consisting of a single nucleotide mutation at a specific locus. SNP genotyping has proven very useful in identifying disease-related mutations in various species, and as a result many techniques for SNP detection have been developed. 

In this application note, we demonstrate how SpectraMax microplate readers can be used to read final KASP products using a KASP assay validation kit from LGC Genomics. 

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