Molecular Devices

Application Note: Normalize HTRF cytokine assays to cell viability

Pro- and anti-inflammatory cytokines play a central role in autoimmunity and inflammatory and infectious diseases. They are also key players in metabolic disorders and oncology, particularly the anti-tumor immune response. Homogeneous Time Resolved Fluorescence (HTRF) from Cisbio Bioassays provides a versatile platform for the quantitation of various cytokines & chemokines.

Here, we demonstrate how to perform a relevant analysis of cytokine secretion along with cell viability, using the SpectraMax i3x® Multi-Mode Microplate Reader with SpectraMax® Minimax® 300 Imaging Cytometer.

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