Molecular Devices

Application Note: Normalize HTRF cytokine assays to cell viability

Pro- and anti-inflammatory cytokines play a central role in autoimmunity and inflammatory and infectious diseases. They are also key players in metabolic disorders and oncology, particularly the anti-tumor immune response. Homogeneous Time Resolved Fluorescence (HTRF) from Cisbio Bioassays provides a versatile platform for the quantitation of various cytokines & chemokines.

Here, we demonstrate how to perform a relevant analysis of cytokine secretion along with cell viability, using the SpectraMax i3x® Multi-Mode Microplate Reader with SpectraMax® Minimax® 300 Imaging Cytometer.

Please complete the form below and a link to the application note will be emailed to you.

 

By submitting your details, you confirm that you have reviewed and agree with the Molecular Devices Privacy Policy, and that you understand your privacy choices as they pertain to your personal data as provided in the Privacy Policy under “Your Privacy Choices”.