Molecular Devices

Detect inflammatory cytokines at picogram levels with Sword ELISA Boosters

Traditional ELISA methods using the horseradish peroxidase (HRP) substrate 3,3’,5,5’- tetramethylbenzidine (TMB) often fail to detect low abundance analytes, such as inflammatory cytokines. The Sword ELISA Booster, a next-generation ELISA detection technology that directly replaces the detection reagents in a traditional, gold-standard ELISA, increases sensitivity up to 30 fold.

This enhanced assay allows you to detect lower levels of these cytokines and advance your understanding of diseases associated with these mediators of inflammation. Here, we demonstrate how three SpectraMax® microplate readers we tested met the criteria for sensitivity outlined for the Sword ELISA Boosters.

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