Molecular Devices

Application Note: Measure intrinsic tryptophan fluorescence
on the SpectraMax iD3 microplate reader

Characterize proteins by measuring intrinsic tryptophan fluorescence on the SpectraMax® iD3 Multi-Mode Microplate Reader. Using 15 nm excitation and 25 nm emission bandwidths, the reader can detect tryptophan in the low nM range. The SpectraMax iD3 reader and SoftMax® Pro Software enable maximum sensitivity by determining the optimal excitation and emission wavelengths. Changes in the peak fluorescence emission wavelength indicative of protein folding and denaturation can be automatically identified by the software.

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